A textbook of general botany by Gilbert Morgan Smith; et al

By Gilbert Morgan Smith; et al

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In fact, plant cell cultures are now being grown in disposable bioreactors with volumes up to 400 L. This trend has been witnessed for both the development and commercial manufacture of therapeutic proteins, secondary metabolite-based pharmaceuticals and cosmetic compounds. Prominent examples of commercial products are Protalix’s ELELYSO and Mibelle Biochemistry’s Phyto Cell Tech-derived bioactive compounds. This chapter discusses the current state of disposable bioreactor technology for plant cell cultures.

This was expected due to the comparability of the engineering data for both benchtop bioreactor versions, as proven by Kaiser et al. [89]. The UniVessel SU Bioreactor operated with a rigid single-use vessel (2 L filling volume) in which two-stage segment blade stirrers, an L-shaped macrosparger and a standard sensor were mounted. Previously realized CFD simulations confirm the presence of an axial flow pattern with downward pumping discharges from the stirrers at clockwise rotation [90]. Mixing times are dependent on P/V and filling volume.

It was carried out by using an Integrity Wand Mixer from ATMI (now a part of Pall Life Sciences) at bench top scale. The positive results can be taken as basis for further studies with corresponding bioreactor systems, such as the Nucleo Bioreactor and the Pad-drive Bioreactor. Both bioreactor systems differ only in the fact that their control units have a single-use cube-shaped bag with a paddle-shaped mixing which performs an elliptical rotating motion. The cultivation bag also includes a micro-sparger that is fixed at the mixing device and ensuring kLa values up to 200 h−1 [91].

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