Assessment of Immune Status by the Leukocyte Adherence by D. M. P. Thomson

By D. M. P. Thomson

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This is an important approach because contact sensitivity has been studied extensively by many relatively conventional techniques. Two aspects of contact sensitivity are of special relevance. First, it is T lymphocyte dependent (Cooper and Ada, 1972); and second, the model has been well defined with respect to its specificity for various simple compounds. This specificity tends to be narrowly restricted to the immunizing substances (Silverstein and Gell, 1962; Fleischman and Eisen, 1975), recognizing both protein carriers and also attached haptenic groupings.

LAI-reactive cell populations in the mouse have been investigated by Creemers (1977). The work led to an interesting set of conclusions as to the nature of cellular interactions in the microplate assay. Creemers immunized mice with a purified preparation of mammary tumor virus (MTV) and was able to generate highly specific LAI reactions and specific LAIF production by spleen cells from the immunized recipients. LAIF production was eliminated by removing T cells from the splenic mixture, but T cell-enriched populations were unable to react unless nylon-adherent cells were added back to the mixture.

43, 141-149. Halliday, W. , Halliday, J. , Campbell, C. , Maluish, A. , and Powell, L. W. (1974a). Brit. Med. J. 2, 349-352. Halliday, W. , Maluish, A. , and Isbister, W. H. (1974b). Br. J. Cancer 29, 31-35. Halliday, W. , Maluish, A. , and Miller, S. (1974c). Cell Immunol. 10, 467-475. Halliday, W. , Maluish, A. , Little, J. , and Davis, N. C. (1975). Int. J. Cancer 16, 645-658. Halliday, W. , Maluish, A. , Stephenson, P. , and Davis, N. C. (1977). Cancer Res. 37, 1962-1971. Halliday, W. , Koppi, T.

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